Composite

Part:BBa_K759012:Design

Designed by: Kenta Mine   Group: iGEM12_HokkaidoU_Japan   (2012-09-20)


eCFP + E. coli aggregation (Ag43) device induced by pBAD promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2694
    Illegal NgoMIV site found at 3918
    Illegal NgoMIV site found at 4428
    Illegal NgoMIV site found at 4449
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3444
    Illegal AgeI site found at 4188
    Illegal AgeI site found at 4602
    Illegal AgeI site found at 4844
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

6 PstI sites exists in Ag43 coding site so we assembled this composite part without using PstI.


Source

Ag43(BBA_K346007) was derived from E. coli K-12 strain by Peking 2010 iGEM.

References