Composite
Part:BBa_K759012:Design
Designed by: Kenta Mine Group: iGEM12_HokkaidoU_Japan (2012-09-20)
eCFP + E. coli aggregation (Ag43) device induced by pBAD promoter.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2694
Illegal NgoMIV site found at 3918
Illegal NgoMIV site found at 4428
Illegal NgoMIV site found at 4449
Illegal AgeI site found at 979
Illegal AgeI site found at 3444
Illegal AgeI site found at 4188
Illegal AgeI site found at 4602
Illegal AgeI site found at 4844 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
6 PstI sites exists in Ag43 coding site so we assembled this composite part without using PstI.
Source
Ag43(BBA_K346007) was derived from E. coli K-12 strain by Peking 2010 iGEM.